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Registros recuperados : 5 | |
1. | | AREVALO, A.P.; BASIKA, T.; ANCHETA, S.; PERELMUTER, K.; RICCIARDI, A.; BOLLATI-FOGOLÍN, M.; CRISPO, M. 3R's applied to in vivo biological activity of recombinant human erythropoietin assay. Technical Article. Biological Models Research and Technology Journal. 2023, Volume 3, Issue 1, e00012023. http://dx.doi.org/10.4322/2675-9225.00012023 -- OPEN ACCESS. Article history: Submitted date 9 February 2023, Accepted date 19 April 2023. -- Correspondence: Crispo, M.; Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; email:crispo@pasteur.edu.uy --...Biblioteca(s): INIA Las Brujas. |
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2. | | CIUFFO, C.; ARÉVALO, A.P.; GARCIA, F.; PERDOMO, T.; CRISPO, M.; GIANNITTI, F.; BUSCHIAZZO, A.; ZARANTONELLI, L. Estudio y caracterización de una variante autóctona de Leptospira para su potencial uso como antígeno en vacunas de uso veterinario. In: Jornadas Uruguayas de Buiatría, 49., 2022. Paysandú, Uruguay: Centro Médico Veterinario de Paysandú; Filial de la Sociedad de Medicina Veterinaria del Uruguaya; Sociedad Uruguaya de Buiatría, 9 al 11 Junio 2022. p.192-194. p.192-194.Biblioteca(s): INIA Las Brujas. |
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3. | | CIUFFO, C.; ARÉVALO, A.P.; GARCIA, F.; PERDOMO, T.; CRISPO, M.; GIANNITTI, F.; BUSCHIAZZO, A.; ZARANTONELLI, L. Estudio de la capacidad infectiva, colonización renal e inmunogenicidad de Leptospira interrogans serogrupo Pomona serovar Kennewicki en un modelo murino sub-letal y crónico. [abstract]. Sub-área 4: Patogenia, virulencia y resistencia. In: Anais III Consensos Latinoamericanos em Leptospirose Animal. Nov 30-Dic 02, 2021 (evento online).Biblioteca(s): INIA Las Brujas. |
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4. | | ALTIER, N.; ABREO, E.; BEYHAUT, E.; GARAYCOCHEA, S.; TORRES, P.; CERECETTO, V.; MARTÍN, N.; CUITIÑO, M.J.; CRISPO, M.; ARÉVALO, A.P.; REGO, N.; ARROSPIDE, G.; LAGE, M.; SUNDBERG, G. Desarrollo de un biofertilizante microbiano para aumentar la disponibilidad de fósforo en el cultivo de soja. * Sustentabilidad. Revista INIA Uruguay, 2020, no.62, p.95-100. (Revista INIA; 62) *Proyecto: Desarrollo de inoculantes para la movilización de fósforo como insumo en la producción agrícola. INIA/IP/EMPRESAS. Proyecto financiado
por la herramienta Alianzas para la Innovación de la Agencia Nacional de Investigación e...Biblioteca(s): INIA Las Brujas. |
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5. | | CABRERA, A.; FRESIA, P.; BERNÁ, L.; SILVEIRA, C.S.; MACÍAS-RIOSECO, M.; AREVALO, A.P.; CRISPO, M.; PRITSCH, O.; RIET-CORREA, F.; GIANNITTI, F.; GIANNITTI, F.; FRANCIA, M.E.; ROBELLO, C. Isolation and molecular characterization of four novel Neospora caninum strains. Genetics, Evolution, and Phylogeny - Short Communication. Parasitology Research, 1 December 2019, Volume 118, Issue 12, Pages 3535-3542. Doi: 10.1007/s00436-019-06474-9 Article history: Received: 11 April 2019 / Accepted: 24 September 2019 / Published online: 7 November 2019.
Funding Sponsor: Agencia Nacional de Investigación e Innovación (ANII).
Funding Text: This project was funded by grant...Biblioteca(s): INIA Las Brujas. |
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Registros recuperados : 5 | |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
17/04/2024 |
Actualizado : |
17/04/2024 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
AREVALO, A.P.; BASIKA, T.; ANCHETA, S.; PERELMUTER, K.; RICCIARDI, A.; BOLLATI-FOGOLÍN, M.; CRISPO, M. |
Afiliación : |
ANA PAULA AREVALO, Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; TATIANA BASIKA, Unidad Mixta entre Instituto Pasteur de Montevideo e INIA (UMPI), Montevideo, Uruguay; Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; Institut Pasteur de Montevideo, Cell Biology Unit, Montevideo, Uruguay; SERGIO ANCHETA, Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; KAREN PERELMUTER, Institut Pasteur de Montevideo, Cell Biology Unit, Montevideo, Uruguay; ALEJANDRO RICCIARDI; MARIELA BOLLATI-FOGOLÍN, Institut Pasteur de Montevideo, Cell Biology Unit, Montevideo, Uruguay; MARTINA CRISPO, Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay. |
Título : |
3R's applied to in vivo biological activity of recombinant human erythropoietin assay. |
Complemento del título : |
Technical Article. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Biological Models Research and Technology Journal. 2023, Volume 3, Issue 1, e00012023. http://dx.doi.org/10.4322/2675-9225.00012023 -- OPEN ACCESS. |
ISSN : |
2675-9225 |
DOI : |
10.4322/2675-9225.00012023 |
Idioma : |
Inglés |
Notas : |
Article history: Submitted date 9 February 2023, Accepted date 19 April 2023. -- Correspondence: Crispo, M.; Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; email:crispo@pasteur.edu.uy -- FUNDING: This work was supported by FOCEM (MERCOSUR Structural Convergence Fund), COF 03/11 and ANII (EQL_2013_X_1_2). -- Document type: Article Bronze Open Access. --
Publisher: Brazilian Society for Laboratory Animal Science. |
Contenido : |
ABSTRACT.- An enhanced protocol for the evaluation of in vivo biological activity of recombinant human erythropoietin (rhEPO) assay in mice is described, following European Pharmacopoeia (Ph. Eur.) version 10.0 guideline and optimized by applying the principles of Replacement, Reduction and Refinement (3Rs) in animal experimentation. In Laboratory Animal Science, one of the main principles is to optimize the experimental protocols in order to achieve the best results with the lowest number of animals and refine the procedures to avoid unnecessary suffering. The main objective of this protocol is to comply with international guidelines for rhEPO evaluation, applying refinement on procedures and reduction in animal use. Some of the features included in this protocol are the increase in number of rhEPO batches tested simultaneously against an international standard, leading to a substantial reduction in the number of animal's used, and refinement on animal handling techniques for subcutaneous drug administration and blood withdrawal. The implemented improvements were validated by reticulocyte estimation to ensure compliance with international criteria established for this trial and institutional quality management system. © 2023, Brazilian Society for Laboratory Animal Science. All rights reserved. |
Palabras claves : |
3R’s; Biological activity; Erythropoietin; European Pharmacopeia; Mice; UNIDAD MIXTA PASTEUR + INIA. |
Asunto categoría : |
A50 Investigación agraria |
URL : |
https://bmrtsbcaljournal.com/article/10.4322/2675-9225.00012023/pdf/bmrt-3-1-e00012023.pdf
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Marc : |
LEADER 02721naa a2200301 a 4500 001 1064596 005 2024-04-17 008 2023 bl uuuu u00u1 u #d 022 $a2675-9225 024 7 $a10.4322/2675-9225.00012023$2DOI 100 1 $aAREVALO, A.P. 245 $a3R's applied to in vivo biological activity of recombinant human erythropoietin assay.$h[electronic resource] 260 $c2023 500 $aArticle history: Submitted date 9 February 2023, Accepted date 19 April 2023. -- Correspondence: Crispo, M.; Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; email:crispo@pasteur.edu.uy -- FUNDING: This work was supported by FOCEM (MERCOSUR Structural Convergence Fund), COF 03/11 and ANII (EQL_2013_X_1_2). -- Document type: Article Bronze Open Access. -- Publisher: Brazilian Society for Laboratory Animal Science. 520 $aABSTRACT.- An enhanced protocol for the evaluation of in vivo biological activity of recombinant human erythropoietin (rhEPO) assay in mice is described, following European Pharmacopoeia (Ph. Eur.) version 10.0 guideline and optimized by applying the principles of Replacement, Reduction and Refinement (3Rs) in animal experimentation. In Laboratory Animal Science, one of the main principles is to optimize the experimental protocols in order to achieve the best results with the lowest number of animals and refine the procedures to avoid unnecessary suffering. The main objective of this protocol is to comply with international guidelines for rhEPO evaluation, applying refinement on procedures and reduction in animal use. Some of the features included in this protocol are the increase in number of rhEPO batches tested simultaneously against an international standard, leading to a substantial reduction in the number of animal's used, and refinement on animal handling techniques for subcutaneous drug administration and blood withdrawal. The implemented improvements were validated by reticulocyte estimation to ensure compliance with international criteria established for this trial and institutional quality management system. © 2023, Brazilian Society for Laboratory Animal Science. All rights reserved. 653 $a3R’s 653 $aBiological activity 653 $aErythropoietin 653 $aEuropean Pharmacopeia 653 $aMice 653 $aUNIDAD MIXTA PASTEUR + INIA 700 1 $aBASIKA, T. 700 1 $aANCHETA, S. 700 1 $aPERELMUTER, K. 700 1 $aRICCIARDI, A. 700 1 $aBOLLATI-FOGOLÍN, M. 700 1 $aCRISPO, M. 773 $tBiological Models Research and Technology Journal. 2023, Volume 3, Issue 1, e00012023. http://dx.doi.org/10.4322/2675-9225.00012023 -- OPEN ACCESS.
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